Extraction of essential oil from nigella sativa using supercritical carbon dioxide. Antibacterial activity of total extracts and essential oil. Anticancer activity of nigella sativa black seed a. Nowadays, multiple drug resistance has developed due to indiscriminate use of commercial antimicrobial drugs commonly used in treatment of. Hanene jrah harzallah, bochra kouidhi, guido flamini, amina bakhrouf and touhami mahjoub, chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of tunisian nigella sativa essential oil and thymoquinone, food chemistry, 129, 4, 1469, 2011. Analysis and antibacterial activity of nigella sativa. Gcms analysis of nigella sativa seeds and antimicrobial braz. Composition, in vitro antioxidant and antimicrobial. Fazly bazzaz2, and maryam motevaly haghi3 1correspondence author. Huge number of studies have been carried out in recent years on the pharmacological effects of these seeds and also the possible relationship with their constituents. Essential oil eo of nigella sativa has a broad antimicrobial spectrum. Effect of ethanolic extracts of spices named nigella sativa.
Nigella sativa oil as well as methanolic extract have been found to possess remarkable antibacterial activity against multidrug resistant coagulase negative staphylococci. This study was designed to examine the in vitro antimicrobial activity of nigella sativa seed by the paper disc agar diffusion method. Seed extract of nigella sativa contains various phytochemicals having antibacterial, antifungal and medicinal properties. This study aimed to analyze the antimicrobial activity of black cumin nigella sativa extracts in inhibiting the growth of pathogenic and spoilage bacteria. D ctrd is a leading cause of sexually transmitted diseases. Pdf antimicrobial activity of black cumin extracts.
Journal of ethnopharmacology, 34 1991 27578 elsevier scientific publishers ireland ltd. Abbouni laboratory for molecular microbiology proteomics and health, department of biology, faculty of natural science and life, university of djillali liabes of sidi bel abbes, 22000. The aim of the present research was to find out the antibacterial activity of nigella sativa and piper nigrum against the 2 gram positive and 3 gram negative bacteria. It is a small shrub with tapering green leaves and rosaceous white and purplish flowers. Poonam prakash department of chemistry shiats, allahabad, india abstract. Antimicrobial activity of nigella sativa seed extract. Nigella sativa essential oils with high concentration of 30 ug carvacrol and thymol are more sensible against bacterias then those with lower concentrations 10 ug maybe because they are responsible of the antibacterial activity. Antibacterial activity of thymoquinone, an active principle. Journal of medicinal plants research antibacterial.
The nigella sativa extract prevented bacterial growth. Antimicrobial activity of nigella sativa against bacteria incubator for a day at 37oc. Sultan mohideen abstract plants have been documented for their medicinal potential which is in use by the traditional healers and in indian system of medicine namely, ayurveda, unani and siddha. Its seeds have a great medicinal importance and have been reported to exhibit many pharmacological effects that include antiparasitic, antibacterial, antifungal, antiviral, antioxidant and antiinflammatory activities. Nigella sativa black cumin seed oil and methanolic extract were tested in varying dilutions against strains of staphylococcus epidermidis and other coagulasenegative staphylococci resistant to a number of clinically used antibiotics isolated from. Pharmaceutical research center, faculty of pharmacy, 65.
The strength of inhibition and the spectrum of antimicrobial activity of n. Seed extracts from six species of the genus nigella family ranunculaceaenigella arvensis, nigella damascena, nigella hispanica, nigella nigellastrum, nigella orientalis, and nigella sativaobtained by successive extraction with nhexane, chloroform, and methanol, were tested for their antimicrobial activity against 10 strains of pathogenic bacteria and yeast using the microdilution. Antibacterial and antioxidant activity of nigella sativa. Effect of ethanolic extracts of spices named nigella sativa, allium sativum and syzygium aromaticum against pseudomonas aeruginosa in vitro 11 2 2 3arifa jabeen, quratulain hanif, shehar bano, maryam riaz and ali ahmed sheikh 1department of microbiology, university of veterinary and animal sciences, lahore, pakistan. Nigella sativa ns seeds have been used for medicinal purposes for centuries both as herbs and its oil. Pdf in vitro antimicrobial activity of nigella sativa. Antibacterial activities of the honeys were standardized according to phenol equivalence. For this purpose, extracts of nigella sativa seeds were prepared in ethyl acetate, methanol, acetone, hexane, diethyl ether, chloroform. Black cumin nigella sativa and its constituent thymoquinone. Topozada 1965 reported antibacterial activity of the phenolic fraction of n.
Methanol extract of nigella sativa exhibited significant antibacterial activity at the concentration of 50 mgml p. Sh msu d, 2h f z 3r as e dab u l h 1l ab or ty im u nh ep i cd v, sf b 2d ep ar tm nof m i cb lg y dp s, f u h h s 3d ep artm nof m icb lg y dp h,f u v. The present study was aimed at determining the chemical constituents of nigella sativa seeds and their anticancer and antibacterial activities. Sativa seeds were evaluated by agarwell diffusion method. Detection of the antibacterial effect of nigella sativa ground seeds. In the present study antibacterial effect of aqueous extract of seeds was studied against few of clinical isolates including both gram po results. Nigella sativa is a herbaceous plant found in the middle east, europe and western and middle asia. Thus, this study was undertaken to develop novel gel formulations from seeds of nigella sativa l. Antibacterial and antifungal activity assesment of nigella. Antimicrobial effect of nigella sativa seed extract. Oleoresins have high concentration of unsaturated fatty acids along. New antimicrobial agents are urgently needed to overcome.
Antibacterial activity of the essential oils of nigella sativa l. An in vitro study model, authormd saifur rahman and md. Supercritical fluid extraction, nigella sativa, antimicrobial activity, gram positive and gram negative bacteria, university putra malaysia, yemen introduction r the extraction of essential oil components using. Chemical constituents and antimicrobial and antioxidant. A well was made in another plate which was filled with 40 ul of the solvent only. Chemical composition, antimicrobial potential against. Only nigella sativa extract alone showed synergistic effect with antibiotics and the positive results were showed by s.
Antibacterial activity of honey and nigella sativa l. The antimicrobial activity from ethanol and petroleum ether extracts against multiple microorganisms was re ported 8. Moreover, significant antimicrobial activity against multidrugresistant clinical bacterial isolates has been also reported. Pdf antibacterial activity of nigella sativa and piper. An alarming increase in bacterial strains resistant to existing antimicrobial agents demands a renewed effort to seek agents effective against pathogenic bacteria resistant to current antimicrobials. Its ripe fruit contains tiny seeds, dark black in color, known as habba alsauda or habba albarakah in arabic and black seed in. The antimicrobial effects of nigella sativa have extensively been studied in vitro 15,16, 29, 31,43,60,74 and in vivo 66 against various. Phytochemical analysis of nigella sativa and its antibacterial and anticancer activities muqdad khairi yaheya dr.
Analysis and antibacterial activity of nigella sativa essential oil formulated in microemulsion system j. Nigella sativa oil was then studied for antibacterial activity against these multidrug resistant isolates of staphylococcus aureus in varying. In the present study, an excellent activity of the n. Comparison of chemical composition and antibacterial. The extract was prepared by reflux extraction method. Pdf antimicrobial activity of nigella sativa seed extract aktiviti. Mohd tariq salman1, ra khan1, i shukla2 departments of pharmacology1 and microbiology2, jawaharlal nehru medical. A modified disc diffusion method was used for determination of the antibacterial activities of the nigella sativa ground seeds with water nsgsw, and the following steps were carried out. Dermatological effects of nigella sativa sciencedirect. Studies on the antimicrobial activity of nigella sativa. Antimicrobial activity of nigella sativa seed extract aktiviti antimikrob ekstrak nigella sativa article pdf available in sains malaysiana 422. Formulation, characterization and antimicrobial properties of. Pdf evaluation of antibacterial activity of piper betel.
Extraction of essential oil from nigella sativa using. Samanthi evaluation of phytochemical and antibacterial. Filter paper discs impregnanted with the diethyl ether extract of nigella sativa seeds 25400. Hence, the present study was deliberately aimed with a nanoparticles using seeds of nigella sativa as a reducing. Alteration in antibacterial potential of nigella sativa l. The antimicrobial properties of herbal plants and their extracts have been recognized since ancient times, while attempts to illustrate these.
Detection of the antibacterial effect of nigella sativa. Antimicrobial activity of black cumin seed nigella sativa l. Evaluation of antimicrobial and antiinflammatory activities. The aqueous and organic extracts of different germinating stages of nigella sativa seeds were. Petri dishes with 20 ml of mueller hinton agar were prepared and streaked with bacterial strains. Antiinflammatory, analgesic and antipyretic activity of nigella sativa. In vitro antibacterial activity of the volatile oil of.
Effect of nigella sativa the black seed on immunity. Antibacterial effect of turkish black cumin nigella sativa l. Pdf abstract pathogenic bacterial infections have become a major health problem worldwide. Nigella sativa, antibacterial activity, gcms introduction infectious diseases pose serious problems to health and they are main cause of morbidity and mortality worldwide1. Antimicrobial activity of black cumin seeds nigella sativa.
Antiyeast activity of each extract was checked by agar well diffusion method against saccharomyces. Essential oils extracted from five fiber varie ties of. The in vivo antimicrobial effect of the nigella sativa seeds extract at a concentration of 33% on pustules staphylococcal skin infections was assessed and compared with standard drug mupirocin on 40 neonates. Nigella sativa extract showed antibacterial synergism with streptomycin and gentamicin. Apr, 2011 thymoquinone is an active principle of nigella sativa seed known as habbah alsauda in arabic countries and sinouj in tunisia.
For this purpose, extracts of nigella sativa seeds were. We investigate in this work the chemical composition by gceims, the antibacterial and the cytotoxic activities of tunisian nigella sativa essential oil and its bioactive compound, thymoquinone, were tested against various clinical cariogenic bacteria n 30. The antimicrobial and resistance modifying activities of. The aim of this study was to evaluate the antimicrobial activity of the bioactive compounds pcymene, thymoquinone, carvacrol, and thymol of n. Exploring antiyeast activity of nigella sativa seed.
Pathogenic bacterial infections have become a major health problem worldwide. Antibacterial activity of thymoquinone and thymohydroquinone. Mics of garanda, black seed and tualang honey ranged from 5. Exhibiting good antimicrobial activity, nigella sativa l. New antimicrobial agents are urgently needed to overcome this problem. Dec 30, 2010 nigella sativa ns seeds have been used for medicinal purposes for centuries both as herbs and its oil. Nigella sativa seeds contain 3638% fixed oils, proteins, alkaloids, saponin and 0. This study is designed to explore antimicrobial effect of extract from the nigella sativa seeds against skin pustules infection. In vitro antimicrobial activity of ethanolic seeds extracts of nigella sativa linn in the sudan. Disc diffusion and in agar dilution methods were performed to assess the antibacterial activity. Focus on antimicrobial effects, different extracts of n. Antibacterial activity of the essential oils of nigella. All neonates were divided and examined into two experimental and control groups randomly. Pdf in vitro antimicrobial activity of ethanolic seeds.
Antimicrobial activity of black cumin seeds nigella. This study was designed to evaluate the antibacterial activity of algerian honey and some extracts of nigella sativa l. Known by the common name black cumin is a medicinal plant from the ranunculaceae family. Ranunculaceae, commonly known as black seed or black cumin has been used for medicinal purposes for.
Gcms analysis of nigella sativa seeds and antimicrobial. Mohd tariq salman1, ra khan1, i shukla2 departments of pharmacology1 and microbiology2, jawaharlal nehru medical college, aligarh muslim university, aligarh 202002. The agar well diffusion method was used to test the antibacterial activity of nigella sativa l. In this study several seed extracts from nigella sativa l. Chloroform and ethanol extracts of black cumin seeds nigella sativa were analyzed for antibacterial activity against five food and water borne pathogens, namely, staphylococcus aureus, bacillus cereus, vibrio cholerae, klebsiella pneumoniae, escherichia coli, and one food spoilage bacteria bacillus subtilis, all of which were previously found to be resistant to different antibiotics. The antibacterial activity of thymoquinone tq and its biofilm inhibition potencies were investigated on 11 human pathogenic bacteria. Our findings were in agreement with literature reporting thymoquinone, the most active constituent, to have substantial antimicrobial activity against mrsa. Pdf antibacterial activity of nigella sativa seed extracts. Conclusion it may be concluded from this study that n. Widely used in traditional medicine and as a food condiment in the arab world. Consequently, black cumin oil may be used as an antimicrobial agent in food products to prevent spoilage. The present research was carried out in the microbiology research laboratory. Development of novel topical cosmeceutical formulations.
Studies on the antimicrobial activity of nigella sativa seed black. Some investigations performed to show the possibility of antimicrobial and antibacterial activities of these seeds using their extracts or oil ali. Development of novel topical cosmeceutical formulations from. Antimicrobial activity of nigella sativa seed extract ukm.
Evaluation of phytochemical and antibacterial activity of nigella sativa l. Bioactive compounds of nigella sativa essential oil as. Graph 1,2,3,4 and fig 1,2 nigella sativa essential oils methanol extracts have more antibacterial and antifungal properties than etheric ones. Pdf antimicrobial activity of black cumin seeds nigella. Aug 31, 2017 harzallah hj, bochra k, guido f, amina b, touhami m 2011 chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of tunisian nigella sativa essential oil and thymoquinone. The equipment used were cleaned and sterilized by immersing them in ethanol for 10 min then washed with distill waterd.
Production of new potent agents is urgently needed, especially for hospitals and health care centers. Seeds of nigella sativa have been used for a long time in folk medicine for the treatment of skin infections. Volume 34, issues 23, september 1991, pages 275278. The aim of the study was to evaluate the chemical composition and antibacterial activity of the oil of nigella sativa black seed against escherichia coli o157. A well was made in another plate which was filled with 40 ul of ampicillin as a positive control and incubated in similar ways. The results provide justification for the use of the plants in folk medicine to treat various infectious diseases. The antibacterial activity of seed extracts was initially screened against s. Gas chromatography mass spectrometry was run to specify their chemical composition. Hatem departments of pharmacology and microbiology, faculty of veterinary medicine, cairo university, cairo. Thymoquinone, pcymene monoterpene, longifolene sesquiterpene, and thymohydroquinone were responsible for strong antimicrobial activity of black cumin oil. Antimitotic and antibacterial effects of the nigella. Studies on the antimicrobial activity of nigella sativa seed.
But till date, up to our knowledge, there is no report on synthesis of silver nanoparticles from nigella sativa. In this study, antibacterial activity of nigella sativa seed extract against some pathogenic bacterial strains streptococcus pyogene, pseudomonas aeruginosa, klebseilla pneumoniae and proteus vulgaris was evaluated. Antimicrobial activity of black cumin seeds nigella sativa against multidrug resistant strains of coagulase negative staphylococci. Their antiyeast properties have not yet been investigated thoroughly.
The effect of nigella sativa extract, on streptococcus faecalis, escherichia. Evaluation of the antimicrobial activity of aqueous. Thymoquinone is an active principle of nigella sativa seed known as habbah alsauda in arabic countries and sinouj in tunisia. Pdf wide spectrum antibacterial activity of nigella sativa l. Bacterial biofilms tend to exhibit significant tolerance to antimicrobials drugs during infections. Comparison of chemical composition and antibacterial activity of nigella sativa seed essential oils obtained by different extraction methods l. Wide spectrum antibacterial activity of nigella sativa l. All media plates 9 cm in diameter were prepared with mullerhinton agar. Evaluation of the synergistic antimicrobial effect of. In islam it is regarded as one of the greatest forms of healing medicine included in the medicine of prophet mohammed.
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